PCR on Microfluidic Chips

The standard procedure for identifying traces of nucleic acid involves quantitative detection after target DNA has been amplified to a predetermined level using PCR. The most traditional method for amplifying DNA, PCR is typically performed in large-scale laboratory cycles and has uses in cloning, sequencing, and diagnostics. These days, the procedure can be streamlined onto microfluidic chips, which lowers the price and consumption of biological sample production as well as the length of time needed for DNA amplification. Additionally, it is possible to link chip-based microfluidic devices with additional stages of DNA processing and analysis. Thus, to obtain ultrasensitive genetic analysis in the domains of molecular biology and clinical diagnostics, microfluidic chips integrated with PCR-based amplification can be widely utilized.